Lighthouse Proteomics

Proteomics Services

PTM Characterization

Protein post-translational modifications can modulate the range of activities and alter the interactions of proteins within a cell’s proteome. Protein PTMs allow for a variety of modified cell function including varied signaling processes, cellular differentiation, and gene expression regulation. Modifications such as phosphorylation, acetylation, methylation, and ubiquitination are added or removed from key proteins in response to disease states or drug treatment conditions. A critical step in understanding these dynamic changes within the proteome is to establish a routine method to access and identify the wide variety of PTMs that are present in the cell.

Detection of PTMs is challenging due to their low frequency and relative abundance, making identification protein PTMs from native samples very difficult. However, utilizing optimized immuno-affinity capture methods coupled with the high sensitivity and specificity of current LC-MS/MS technology, PTM characterization of complex samples can be routinely performed.

Our Protein PTM Characterization service is designed for the identification of proteins and their corresponding PTMs from either a gel band which is cut from a native or denatured PAGE gel or from a purified protein purification method whereby the protein of interest is provided in an elution buffer or bound directly to an affinity capture bead. The soluble protein(s) is digested with trypsin (or other protease) to produce peptide fragments that are then analyzed with LC-MS/MS to compare with a species-specific protein databases for corresponding peptide/protein assignments and corresponding PTMs.

How Can Lighthouse Proteomics Assist You With Your Research?

  • Identify PTMs associated with a purified target protein of interest
  • Investigate PTMs to target protein purified from different sample conditions
You Provide: Purified protein in solution or on affinity beads, a gel slice
We Provide: Protein identifications from protease treated sample using high resolution LC-MS/MS technology
You Receive: A data package will be provided electronically in the form of an XLS result table(s) and a PowerPoint summary report. The data package is also accompanied with a consultation to review the results with you and address any questions you have about the analysis.
Our Proteome-Wide PTM Characterization service is designed for the identification of proteins and their corresponding PTMs from either a cell culture or tissue sample. A denaturing protein lysate is generated from the sample and the resulting protein(s) are digested with trypsin (or other protease) to produce peptide fragments that are then analyzed with LC-MS/MS to compare with a species-specific protein databases for corresponding peptide/protein assignments and corresponding PTMs.

How Can Lighthouse Proteomics Assist You With Your Research?

  • Characterize proteins and associated PTMs related to cell phenotype
  • Identify unique proteins and associated PTMs related to treatment condition
You Provide: flash frozen tissue, cell culture slurry or cell pellet
We Provide: Protein identifications from protease treated sample using high resolution LC-MS/MS technology
You Receive: A data package will be provided electronically in the form of an XLS result table(s) and a PowerPoint summary report. The data package is also accompanied with a consultation to review the results with you and address any questions you have about the analysis.

PTMs include: Phosphorylation: S, T, Y; Acetylation; Succininylation; Methylation & Ubiquitination
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